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Novel Biocatalysts for the Production of
Glycosides
An overview of the glycosides that can be produced with our technology can be found in the Novosides Glycosylation database.
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JOSE DA, ELSTNER M, SCHILLER A (2013) Allosteric Indicator Displacement Enzyme Assay for a Cyanogenic Glycoside. Chem. Eur. J. 19, 14451–14457
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TIMM M, GÖRL J, KRAUS M, KRALJ S, HELLMUTH H, BEINE R, BUCHHOLZ K, DIJKHUIZEN L, SEIBEL J (2013) An Unconventional Glycosyl Transfer Reaction: Glucansucrase GTFA Functions as an Allosyltransferase Enzyme. Chembiochem, 14(18):2423-2426
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DOBRUCHOWSKA JM, MENG X, LEEMHUIS H, GERWIG GJ, DIJKHUIZEN L, KAMERLING JP (2013) Gluco-oligomers initially formed by the reuteransucrase enzyme of Lactobacillus reuteri 121 incubated with sucrose and malto-oligosaccharides. Glycobiology, 23(9):1084-1096
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DE WINTER K, SIMČÍKOVÁ D, SCHALCK B, WEIGNEROVÁ L, PELANTOVA H, SOETAERT W, DESMET T, KŘEN V (2013) Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus. Bioresource Technology, 147:640-644
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VERHAEGHE T, DIRICKS M, AERTS D, SOETAERT W AND DESMET T (2013) Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning. Journal of Molecular Catalysis B: Enzymatic, 96:81-88
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AERTS D, VERHAEGHE T, JOOSTEN HJ, VRIEND G, SOETAERT W, DESMET T (2013) Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input. Biotechnology and Bioengineering. 110(10):2563-2572
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DE WINTER K, VERLINDEN K, KŘEN V, WEIGNEROVÁ L, SOETAERT W AND DESMET T (2013) Ionic liquids as cosolvents for glycosylation by disaccharide phosphorylases: balancing acceptor solubility and enzyme stability. Green chemistry, 15(7):1949-1955
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MRÁZEK H., WEIGNEROVÁ L., BOJAROVÁ P., NOVÁK P., VANĚK O., BEZOUŠKA K. (2013) Carbohydrate synthesis and biosynthesis technologies for cracking of the glycan code: Recent Advances. Biotechnology Advances, 31 (1): 17-37
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DEMO G., FLIEDROVÁ B., WEIGNEROVÁ L., WIMMEROVÁ M. (2013) Crystalization and preliminary X-ray crystallographic analysis of recombinant β-mannosidase from Aspergillus niger. Acta Crystallographica Section F, F69 (3): 288-291
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DESMET T, SOETAERT W, BOJAROVÁ P, KREN V,
DIJKHUIZEN L, EASTWICK-FIELD V, SCHILLER A
(2012) Enzymatic glycosylation of small
molecules: challenging substrates require
tailored catalysts. Chem Eur J, 18:
10786-10801
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ELSTNER M, WEISSHART K, MÜLLEN K & SCHILLER
A (2012) Molecular Logic with a Saccharide
Probe on the Few-Molecules Level, J Am Chem
Soc 134:8098-8100
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WEIGNEROVÁ L, MARHOL P, GERSTORFEROVÁ D &
KŘEN V (2012) Preparatory production of
quercetin-3-β-D-glucopyranoside using
alkali-tolerant thermostable α-L-rhamnosidase
from Aspergillus terreus. Bioresource
Technol 115:222-227
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TRAN GH, DESMET T, SAERENS K, WAEGEMAN H,
VANDEKERCKHOVE S, D'HOOGHE M, VAN BOGAERT I
& SOETAERT W (2012) Biocatalytic production
of novel glycolipids with cellodextrin
phosphorylase. Bioresource Technol 115:84-87
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SCHILLER A (2012) Going beyond glucose
sensing with boronic acid receptors. In:
Molecules at Work (Ed.: B. Pignataro),
Wiley-VCH, Weinheim, 315-338
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DESMET T & SOETAERT W (2012) Broadening the
application potential of disaccharide
phosphorylases through enzyme engineering.
Process Biochem 47:11-17
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SLÁMOVÁ K, BOJAROVÁ P, GERSTORFEROVÁ D,
FLIEDROVÁ B, HOFMEISTEROVÁ J, FIALA M,
POMPACH P, KŘEN V (2012) Sequencing, cloning
and high-yield expression of a fungal β-N-acetylhexosaminidase
in Pichia pastoris. Protein Expr Purif,
82(1):212-217
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KRALJ S, GRIJPSTRA P, VAN LEEUWEN SS,
LEEMHUIS H, DOBRUCHOWSKA JM, VAN DER KAAIJ
RM, MALIK A, OETARI A, KAMERLING JP &
DIJKHUIZEN L (2011)
4,6-α-Glucanotransferase, a novel enzyme
that structurally and functionally provides
an evolutionary link between glycoside
hydrolase enzyme families GH13 and GH70.
Appl Environm Microbiol 77: 8154-63
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SCHILLER A, VILOZNY B, WESSLING RA &
SINGARAM B (2011) Going beyond continuous
glucose monitoring with boronic acid
appended bipyridinium salts. Rev
Fluorescence 2009:155–199
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BOJAROVÁ
P, KŘEN V (2009) Glycosidases: a key to
tailored carbohydrates. Trends Biotechnol,
27(4):199-209
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KŘEN
V (2008) Glycoside vs. Aglycon: The role of
glycosidic residue in biological activity.
In: Glycoscience. Fraser-Reid B, Tatsuta K,
Thiem J (Eds), 2589-2644
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LEEMHUIS H, KELLY RM, DIJKHUIZEN L (2009)
Directed evolution of enzymes: Library
screening strategies. IUBMB Life,
61(3):222-228
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VAN HIJUM SAFT, KRALJ S, OZIMEK LK,
DIJKHUIZEN L, VAN GEEL-SCHUTTEN GH (2006)
Structure-function relationships of
glucansucrase and fructansucrase enzymes
from lactic acid bacteria. Microbiol Mol
Biol Rev 70:157-176
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VILOZNY B, SCHILLER A, WESSLING RA, and
SINGARAM B (2009) Enzyme assays with boronic
acid appended bipyridinium salts. Anal Chim
Acta 649:246-251
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