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Novel Biocatalysts for the Production of Glycosides

An overview of the glycosides that can be produced with our technology can be found in the Novosides Glycosylation database.


  • JOSE DA, ELSTNER M, SCHILLER A (2013) Allosteric Indicator Displacement Enzyme Assay for a Cyanogenic Glycoside. Chem. Eur. J. 19, 14451–14457

  • TIMM M, GÖRL J, KRAUS M, KRALJ S, HELLMUTH H, BEINE R, BUCHHOLZ K, DIJKHUIZEN L, SEIBEL J (2013) An Unconventional Glycosyl Transfer Reaction: Glucansucrase GTFA Functions as an Allosyltransferase Enzyme. Chembiochem, 14(18):2423-2426

  • DOBRUCHOWSKA JM, MENG X, LEEMHUIS H, GERWIG GJ, DIJKHUIZEN L, KAMERLING JP (2013) Gluco-oligomers initially formed by the reuteransucrase enzyme of Lactobacillus reuteri 121 incubated with sucrose and malto-oligosaccharides. Glycobiology, 23(9):1084-1096

  • DE WINTER K, SIMČÍKOVÁ D, SCHALCK B, WEIGNEROVÁ L, PELANTOVA H, SOETAERT W, DESMET T, KŘEN V (2013) Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus. Bioresource Technology, 147:640-644

  • VERHAEGHE T, DIRICKS M, AERTS D, SOETAERT W AND DESMET T (2013) Mapping the acceptor site of sucrose phosphorylase from Bifidobacterium adolescentis by alanine scanning. Journal of Molecular Catalysis B: Enzymatic, 96:81-88

  • AERTS D, VERHAEGHE T, JOOSTEN HJ, VRIEND G, SOETAERT W, DESMET T (2013) Consensus engineering of sucrose phosphorylase: the outcome reflects the sequence input. Biotechnology and Bioengineering. 110(10):2563-2572

  • DE WINTER K, VERLINDEN K, KŘEN V, WEIGNEROVÁ L, SOETAERT W AND DESMET T (2013) Ionic liquids as cosolvents for glycosylation by disaccharide phosphorylases: balancing acceptor solubility and enzyme stability. Green chemistry, 15(7):1949-1955

  • MRÁZEK H., WEIGNEROVÁ L., BOJAROVÁ P., NOVÁK P., VANĚK O., BEZOUŠKA K. (2013) Carbohydrate synthesis and biosynthesis technologies for cracking of the glycan code: Recent Advances. Biotechnology Advances, 31 (1): 17-37

  • DEMO G., FLIEDROVÁ B., WEIGNEROVÁ L., WIMMEROVÁ M. (2013) Crystalization and preliminary X-ray crystallographic analysis of recombinant β-mannosidase from Aspergillus niger. Acta Crystallographica Section F, F69 (3): 288-291

  • DESMET T, SOETAERT W, BOJAROVÁ P, KREN V, DIJKHUIZEN L, EASTWICK-FIELD V, SCHILLER A (2012) Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts. Chem Eur J, 18: 10786-10801

  • ELSTNER M, WEISSHART K, MÜLLEN K & SCHILLER A (2012) Molecular Logic with a Saccharide Probe on the Few-Molecules Level, J Am Chem Soc 134:8098-8100

  • WEIGNEROVÁ L, MARHOL P, GERSTORFEROVÁ D & KŘEN V (2012) Preparatory production of quercetin-3-β-D-glucopyranoside using alkali-tolerant thermostable α-L-rhamnosidase from Aspergillus terreus. Bioresource Technol 115:222-227

  • TRAN GH, DESMET T, SAERENS K, WAEGEMAN H, VANDEKERCKHOVE S, D'HOOGHE M, VAN BOGAERT I & SOETAERT W (2012) Biocatalytic production of novel glycolipids with cellodextrin phosphorylase. Bioresource Technol 115:84-87

  • SCHILLER A (2012) Going beyond glucose sensing with boronic acid receptors. In: Molecules at Work (Ed.: B. Pignataro), Wiley-VCH, Weinheim, 315-338

  • DESMET T & SOETAERT W (2012) Broadening the application potential of disaccharide phosphorylases through enzyme engineering. Process Biochem 47:11-17

  • SLÁMOVÁ K, BOJAROVÁ P, GERSTORFEROVÁ D, FLIEDROVÁ B, HOFMEISTEROVÁ J, FIALA M, POMPACH P, KŘEN V (2012) Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosaminidase in Pichia pastoris. Protein Expr Purif, 82(1):212-217

  • KRALJ S, GRIJPSTRA P, VAN LEEUWEN SS, LEEMHUIS H, DOBRUCHOWSKA JM, VAN DER KAAIJ RM, MALIK A, OETARI A, KAMERLING JP & DIJKHUIZEN L (2011) 4,6-α-Glucanotransferase, a novel enzyme that structurally and functionally provides an evolutionary link between glycoside hydrolase enzyme families GH13 and GH70. Appl Environm Microbiol 77: 8154-63

  • SCHILLER A, VILOZNY B, WESSLING RA & SINGARAM B (2011) Going beyond continuous glucose monitoring with boronic acid appended bipyridinium salts. Rev Fluorescence 2009:155–199

  •  BOJAROVÁ P, KŘEN V (2009) Glycosidases: a key to tailored carbohydrates. Trends Biotechnol, 27(4):199-209

  •  KŘEN V (2008) Glycoside vs. Aglycon: The role of glycosidic residue in biological activity. In: Glycoscience. Fraser-Reid B, Tatsuta K, Thiem J (Eds), 2589-2644

  • LEEMHUIS H, KELLY RM, DIJKHUIZEN L (2009) Directed evolution of enzymes: Library screening strategies. IUBMB Life, 61(3):222-228

  • VAN HIJUM SAFT, KRALJ S, OZIMEK LK, DIJKHUIZEN L, VAN GEEL-SCHUTTEN GH (2006) Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria. Microbiol Mol Biol Rev 70:157-176

  • VILOZNY B, SCHILLER A, WESSLING RA, and SINGARAM B (2009) Enzyme assays with boronic acid appended bipyridinium salts. Anal Chim Acta 649:246-251

December 2010 – November 2014
KBBE.2010.3.3-01: Robust and novel biocatalysts for industrial applications

 © Ghent University 2011